tuaD [2019-02-22 16:51:40]

UDP glucose 6-dehydrogenase

Locus: BSU35580

Isoelectric point: 6.11

Molecular weight: 49.65 kDa

Protein length: 461 aa

Gene length: 1386 bp

Function: biosynthesis of teichuronic acid

Product: UDP glucose 6-dehydrogenase

Essential: no

E.C.: 1.1.1.22

Synonyms: yvhD

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall synthesis → Biosynthesis of teichuronic acid
• Metabolism → Additional metabolic pathways → Biosynthesis of cell wall components → Biosynthesis of teichuronic acid
• Groups of genes → Phosphoproteins → Phosphorylation on a Tyr residue

Gene

Coordinates: 3,654,139 → 3,655,524

The protein

Catalyzed reaction/ biological activity

• UDP-glucose + 2 NAD⁺ + H₂O = UDP-glucuronate + 2 NADH (according to Swiss-Prot)

Protein family

• UDP-glucose/GDP-mannose dehydrogenase family (according to Swiss-Prot)

Paralogous protein(s)

• Ugd, YtcA

Modification

• phosphorylation on a Tyr residue by PtkA PubMed, dephosphorylated by PtpZ PubMed

Effectors of protein activity

• PtkA-dependent phosphorylation stimulates TuaD activity PubMed

Structure

• 3GG2 (48% identity)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: tuaA/2-tuaA/1-tuaB-tuaC-tuaD-tuaE-tuaF-tuaG-tuaH
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigF: sigma factor, PubMed, in SigF regulon

  Regulatory mechanism

  • PhoP: activation, PubMed, in PhoP regulon

  Regulation

  • expressed during sporulation in the forespore (SigF) PubMed

Biological materials

Mutant

• BKE35580 (ΔtuaD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACAGCGCTCAACCCTCTCC, downstream forward: _UP4_TAAAAAGAAAAAGTGTTACT
• BKK35580 (ΔtuaD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACAGCGCTCAACCCTCTCC, downstream forward: _UP4_TAAAAAGAAAAAGTGTTACT

References

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